Isolation and Identification of Aflatoxin and Ochratoxin Producing Fungi from Some Local Food Items  in the Markets of Karbala Governorate

Haider Jawad Abd AL-Kadhim, Zainab Ali Hussein, Jumana Hussein Rashid; Ibrahim Faisal Shalal

Authors

Haider Jawad Abd AL-Kadhim, Zainab Ali Hussein, Jumana Hussein Rashid University of Karbala, College of Applied Medical Sciences, Department of Environmental Health , Iraq
Ibrahim Faisal Shalal University of Karbala, College of Applied Medical Sciences, Department of Pathological Analysis, Iraq

Keywords:

Aflatoxin, Ochratoxin

Abstract

This study showed the isolation and identification of fungi contaminating some foodstuffs and their ability to produce Aflatoxin and Ochratoxin toxins. The results showed the isolation of 17 isolates of fungi isolated from different food sources, which are 10 isolates of Aspergillus niger in the samples red pistachio, basmati rice, amber rice, Nestle and corn and 4 Aspergillus flavus isolates in noodles, soybean, white pistachio and corn samples. One isolate of Aspergillus fumigates in a sunflower seed sample and one isolate of Rhizopus sp. in the biscuit sample and one isolate of Penicillium sp in the red pistachio sample.

Thin plate chromatography test TLC showed that Aspergillus niger isolated from red pistachios produced Ochratoxin and Aflatoxin together, while samples of soybean rice and amber rice produced only Ochratoxin, and Aspergillus flavus isolated from soybean produced Aflatoxin and Ochratoxin from maize. It was an Aflatoxin producer. Penicillium sp isolates isolated from red pistachios were Ochratoxin-producing.

Aim of the study:

A survey study of the fungi in the most traded foodstuffs in the community of Karbala governorate and confirming the most prevalent and polluting fungi in food samples that produce the toxins Aflatoxin and Ochratoxin

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