Antioxidant Scavenging Potential and Screening of Bioactive Constitutes of Trigonella foenumgraecum L. Using GC-MS and FTIR Technique

Saja Ayad Abed Al-Khafaji; Zainab Nadhim Abdul-Kadhim Mahdi; Samah Maytham Abed

Authors

Saja Ayad Abed Al-Khafaji Department of Biology, College of Science for Women, University of Babylon, Iraq
Zainab Nadhim Abdul-Kadhim Mahdi Department of Physics, College of Science for Women, University of Babylon, Iraq
Samah Maytham Abed Department of Chemistry, College of Science for Women, University of Babylon, Iraq

Keywords:

Trigonella foenumgraecum L., GC-MS, FTIR, Antioxidant

Abstract

The diploid, annual plant Trigonella foenum-graecum (TF) L. experiences cleistogamy and is a member of the legume family. Trigonella foenumgraecum L.'s dried, ripe seeds are commonly referred to as fenugreek or just seeds. Its strong aroma makes it a popular ingredient for flavoring cuisine. The primary compounds isolated from Trigonella foenumgraecum L. seeds are linoleic acid, octanoic acid, bis(2-ethylhexyl) ester, carbonic acid, nonanedioic acid, 2,6,6-trimethylcyclohexene-1-methanol, linoleic acid, and 9,12-octadecadienoic acid (Z,Z)-. Ethyl Oleate-d5, 6-isopropylquinoline, dihydrocinchonamine, 4,4-dimethylcyclohexyl) methanamine, vitamin E, and 10-trans,12-cis-Linoleic acid methyl ester are all components. Trigonella foenumgraecum L. fruit extract (methanolic crude extract, ethanol fraction, and standard) and its antioxidant activity, including scavenging of peroxynitrite, hydroxyl, and nitric oxide radicals. Crude, ethyl acetate fraction, ethanol fraction, and standard (712.00±36.14, 656.45±33.00) and Gallic acid (standard) (874.00±38.09) were the different types of extracts that were employed for peroxynitrite scavenging, respectively. Mannitol (standard) has a hydroxyl radical scavenging potential of 520.90±29.81, Benzoic acid (298.71±25.00) and 269.00±22.95 were also noted. The nitric oxide radical scavenging potential for Curcumin (standard) was measured at 42.00±3.05, 25.13±2.98, and 58.07±3.93, in that order.

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References

Giannenas, I.; Sidiropoulou, E.; Bonos, E.; Christaki, E.; Florou-Paneri, P. The history of herbs, medicinal and aromatic plants, and their extracts: Past, current situation and future perspectives. In Feed Additives; Elsevier: Amsterdam, The Netherlands, 2020; pp. 1–18.

Abdallah, E.M.; Alhatlani, B.Y.; de Paula Menezes, R.; Martins, C.H.G. Back to Nature: Medicinal plants as promising sources for antibacterial drugs in the post-antibiotic era. Plants 2023, 12, 3077.

Ahmadi, S.; Ahmadi, G.; Ahmadi, H. A review on antifungal and antibacterial activities of some medicinal plants. Micro Nano Bio Asp. 2022, 1, 10–17.

Al-Snafi, A.E. Therapeutic properties of medicinal plants: A review of their antibacterial activity (Part 1). Int. J. Pharmacol. Toxicol. 2015, 6, 137–158.

Rios, J.-L.; Recio, M.C. Medicinal plants and antimicrobial activity. J. Ethnopharmacol. 2005, 100, 80–84.

Sulieman, A.M.E.; Shaarawy, S.M.; Alghamdi, A.A.; Veettil, V.N.; Abdelgadir, M.; Ibrahim, N.A. Evaluation of antimicrobial and synergistic effects of selected medicinal plants of Hail area with antibiotics. Biosci. Biotechnol. Res. Commun. 2017, 10, 44–50.

Altunta，s, E.; Ozgoz, E.; Ta，ser, O.F. Some physical properties of fenugreek (Trigonella foenum-graceum L.) seeds. J. Food Eng. 2005, 71, 37–43. [CrossRef]

Wani, S.A.; Kumar, P. Fenugreek: A review on its nutraceutical properties and utilization in various food products. J. Saudi Soc. Agric. Sci. 2018, 17, 97–106.

Rashid, F.; Bao, Y.; Ahmed, Z.; Huang, J.-Y. Effect of high voltage atmospheric cold plasma on extraction of fenugreek galactomannan and its physicochemical properties. Food Res. Int. 2020, 138, 109776.

H. Archana and V. G. Bose, “Evaluation of phytoconstituents from selected medicinal plants and its synergistic antimicrobial activity,” Chemosphere, Vol. 8, no. 1. Pp. 113-117, 2022.

S. Wang, X.-Q. Liu, O. Kang, and D. Kwon, “Combination of Sanguisorbigenin and Conventional Antibiotic Therapy for Methicillin-Resistant Staphylococcus aureus: Inhibition of Biofilm Formation and Alteration of Cell Membrane Permeability,” International Journal of Molecular Science, Vol. 23, no. 8. Pp. 4220-4232, 2022.

H. A. H. Alsaidy and A. A. Ali, “Reviews on Plants Gum and Mucilage I: The Composition and Pharmacological Significance of Turmeric (Curcuma longa) Rhizomes Polysaccharides and Mucilage/their Aqueous Extracts Commercial Products,” Univ. Thi-Qar J. Sci., vol. 8, no. 1, pp. 14–25, 2021M. S. Akhtar, M. K. Swamy, and U. R. Sinniah, "Natural bio-active compounds," Natural Bio-Active Compounds, vol. 1, pp. 1-608, 2019.

R. Prasad, S. Acharya, and J. Thomas, “Identification of High Seed Yielding And Stable Fenugreek Mutants” by | Perlego,” 6. 01, 2014.

B. Bin-Hafeez, R. Haque, S. Parvez, S. Pandey, I. Sayeed, and S. Raisuddin, “Immunomodulatory effects of fenugreek (Trigonella foenum graecum L.) extract in mice,” International Immunopharmacology, Vol. 12, no. 4. Pp, 257-265, 2003.

L. a N. Al-Timimi, “Antibacterial and Anticancer Activities of Fenugreek Seed Extract,” Asian Pacific Journal of Cancer Prevention, Vol. 20, no. 12. Pp, 3771, 2019.

T. H. Desai and S. V. Joshi, “Anticancer activity of saponin isolated from Albizia lebbeck using various in vitro models,” Journal of Ethnopharmacology, Vol. 231, no. 6. Pp, 494-502, 2019.

G. K. Devi, K. Manivannan, G. Thirumaran, F. A. A. Rajathi, and P. Anantharaman, “In vitro antioxidant activities of selected seaweeds from Southeast coast of India,” Asian Pacific Journal of Tropical Medicine, Vol. 4, no. 3. Pp, 205-211, 2011.

Beckman JS, Chen H, Ischiropulos H, Crow JP: Oxidative chemistry of peroxynitrite. Methods Enzymol. 1994, 233: 229-240.

Bailly F, Zoete V, Vamecq J, Catteu JP, Bernier JL: Antioxidant actions of ovothiol-derived 4-mercaptoimidazoles: glutathione peroxidase activity and protection against peroxynitrite-induced damage. FEBS Lett. 2000, 486: 19-22.

Aruoma OI, Halliwell B, Hoey BM, Butler J: The antioxidant action of N-acetylcysteine: Its reaction with hydrogen peroxide, hydroxyl radical, superoxide, and hypochlorous acid. Free Rad Biol Med. 1989, 6: 593-597.

Halliwell B, Gutteridge JMC, Aruoma OI: The deoxyribose method: a simple 'test tube' assay for determination of rate constants for reaction of hydroxyl radicals. Anal Biochem. 1987, 165: 215-219.

Miller MJ, Sadowska-Krowicka H, Chotinaruemol S, Kakkis JL, Clark DA: Amelioration of chronic ileitis by nitric oxide synthase inhibition. J Pharmacol Exp Ther. 1993, 264 (1): 11-16.

Balavoine GG, Geletti YV: Peroxynitrite scavenging by different antioxidants. Part 1: convenient study. Nitric oxide. 1999, 3: 40-54.

VRuwali, P.; Pandey, N.; Jindal, K.; Singh, R.V. Fenugreek (Trigonella foenum-graecum): Nutraceutical values, phytochemical, ethnomedicinal and pharmacological overview. S. Afr. J. Bot. 2022, 151, 423–431.

Gavahian, M.; Bannikoppa, A.M.; Majzoobi, M.; Hsieh, C.-W.; Lin, J.; Farahnaky, A. Fenugreek bioactive compounds: A review of applications and extraction based on emerging technologies. Crit. Rev. Food Sci. Nutr. 2024, 64, 10187–10203.

Alu’datt, M.H.; Rababah, T.; Al-ali, S.; Tranchant, C.C.; Gammoh, S.; Alrosan, M.; Ghatasheh, S. Current perspectives on fenugreek bioactive compounds and their potential impact on human health: A review of recent insights into functional foods and other high value applications. J. Food Sci. 2024, 89, 1835–1864.

Sindhusha, V.B.; Rajasekar, A. Preparation and Evaluation of Antimicrobial Property and Anti-inflammatory Activity of Fenugreek Gel Against Oral Microbes: An In Vitro Study. Cureus 2023, 15, e47659.