Preparation and Characterization of Chitosan Nanoparticles from Crab shell and Determination their Activity as Antibacterial and Antioxidant
Keywords:
CS-NPs, Crab shell, Anti-bacterial, antioxidantAbstract
Back ground: Chitosan nanoparticles (CS-NPs) are potential materials for medical application like antibacterial, antioxidant and anticancer. Objective: This study was focused on preparation of CS-NPs from wasted crab shell, determination their antibacterial and antioxidant activity. Methodology: The CS-NPs were prepared by using the ionic gelation method, characterization of CS-NPs includes Scanning Electron Microscopy (SEM) and X-ray Diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR Analysis) and determination their antibacterial against (E. coli and Streptococcus mutanse) and antioxident activity by DPPH test. Results: crystalline nature of the prepared nanoparticles with average size around 32.2 nm scale with rod shape. The synthesized CS-NPs showed antibacterial efficacy against clinical bacterial isolates of MDR in both Gram positive, Streptococcus mutanse, and Gram negative, E. coli By using the disk diffusion method. This activity might be attributed to the unique biological and physicochemical properties of the CS-NPs, which facilitate the disruption of bacterial cell membranes, from results underscore the potential of crab shellas an eco-friendly and efficient biological agent for the synthesis of CS-NPs with potent antibacterial properties. The prepared CS-NPs revealed a significant high antioxidant activity compared to ascorbic acid in the same concentration. Conclusion: It can be concluded that CS-NPs can be prepared from carb sell in an eco-friendly way and this CS-NPs can used as a cost-effective antibacterial agent and antioxidant.
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