UV-visible, Infrared, Fourier transform infrared and Raman spectroscopy, spectrophotometry: Principles and Applications

Abstract

One way to find out how much of a known substance is in an unknown medium is to use spectroscopy, which includes spectrophotometers and other forms of the technique. The most practical and accurate way to analyse unknown samples quantitatively and qualitatively is with spectroscopy. Spectroscopic and spectrophotometric methods of many kinds are invaluable in scientific study for analysing samples down to the sub-ppm level. These methods work on the basic premise that the quantity of a certain kind of radiation—a photon—that a sample absorbs or reflects is proportional to the strength of the incident light at that wavelength. Using these methods, one may quantitatively determine the