Chemical Composition Using FTIR Technique, Antioxidant [Hypochlorous, Hydrogen peroxide and Superoxide Radical Scavenging] and Antimicrobial Activities of Haloxylon salicornicum Methanolic Aerial-Part Extract

Rabab J.H. Al Hasseny; Assist. Prof. Hider M. H. Al-Shirifi; Assist. Prof.  Dr. Hiba Jasim Hamza; Prof. Dr. Abbas. K. Al-Mansoori

Authors

Rabab J.H. Al Hasseny Medical Microbiology, Department of Health Food and Nutrition, College of Food Science, Al-Qasim Green University, Iraq
Assist. Prof. Hider M. H. Al-Shirifi Al-Qasim Green University, Faculty of Environmental Sciences, Environmental health Department, Iraq
Assist. Prof. Dr. Hiba Jasim Hamza University of Babylon, College of Nursing, Medical & Basic Sciences Department, Iraq
Prof. Dr. Abbas. K. Al-Mansoori AL-Qasim Green University, Faculty of Biotechnology, Department of Applied Biotechnology, Iraq

Keywords:

FTIR Technique, Hypochlorous, Hydrogen peroxide, Superoxide Antimicrobial, Haloxylon salicornicum.

Abstract

The objectives of the present study are identification of chemical groups present in Haloxylon salicornicum using a Fourier transform infrared spectrophotometer method and assessment of antioxidant and antimicrobial potentials. The samples H. salicornicum were washed and thereafter air dried to ensure that they were through washed. The mash was filtered employing Whatman filter sheets (no. 1, 125 mm, Cat No. 1061 135 Germany). Approximately 30 mL of each plant extract was taken for this purpose and the concentrations of the final plant extracts were also determined, and all the samples were stored at 4 degrees Celsius. In this case the sample was scanned at infrared region of 400 nm-4000 nm. The human pathogenic bacteria were isolated from Microbiology department of hilla hospital. E.coli, Salmonella, Pseudomonas, Bacillus and Staphylococcus bacteria in particular are known to be part of the major bacterial pathology. Inhibitory zone diameter (mm) were measured after 18–24 h at 37°C. FT-IR peak values of solid analysis according to Type of Intensity, Bond, Type of Vibration and Functional group assignment [Strong, =C–H, Bending, Alkenes] [Strong, C-F, Stretch, alkyl halides][ Medium, C=C, Stretch, Aromatic][ Bending, N-H, Stretch, Amide][ Strong, C=O, Stretch, Ester][ Strong, C-H, Stretch, Alkane]. Antioxidant [Hypochlorous acid, Hydroxyl and Superoxide radical scavenging] Activities of Three fractions of Haloxylon salicornicum methanolic crude extract, namely ethanol fraction and water fraction Aerial-Part Extract have been assayed. Antibacterial activity of secondary metabolites of Haloxylon salicornicum: The inhibition of five pathogenic bacteria and antibiotics by the secondary metabolites of Haloxylon salicornicum (AM-Amikacin; AP-Ampicillin; CF-Cephalothin). Pseudomonas aeruginosa (11.10 ± 0.21), Salmonella enterica (08.39 ± 0.14), Bacillus subtilis (19.00 ± 0.45), Escherichia coli (14.36 ± 0.30) and Staphylococcus aureus (12.00 ± 0.29). Haloxylon salicornicum metabolites was very highly active against Bacillus subtilis (19.00 ± 0.45).

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