Evaluation of the Anti-Oxidant Activity and Screening of Secondary Metabolites Using the FTIR Technique in a Methanolic Seed Extract of Ajwain seeds (Trachyspermum ammi L.)

Rabab J.H. Al Hasseny; Abbas K. Al-Mansoori; Fatima Abd-Al-Radhia, Toqa M.A. Mhayyal

Authors

Rabab J.H. Al Hasseny Medical Microbiology, Department of Health Food and Nutrition, College of Food Science, Al-Qasim Green University, Iraq
Abbas K. Al-Mansoori Department of Genetic Engineering, College of Biotechnology, Al-Qasim Green University, Iraq
Fatima Abd-Al-Radhia, Toqa M.A. Mhayyal AL-Qasim Green University, Faculty of Biotechnology, Department of Medical Biotechnology, Iraq

Keywords:

Anti-Oxidant Activity, Secondary Metabolites, FTIR, Trachyspermum ammi L

Abstract

Ajwain, or Trachyspermum ammi, is a spice with a lot of antioxidant power thanks to the flavonoids and polyphenols it contains in its seeds. Research has revealed that the seeds have strong free radical scavenging capabilities against many types of oxidative stress, mainly due to the presence of thymol, carvacrol, and γ-terpinene. Screening for secondary metabolites and assessing anti-oxidant activity were the goals of this FTIR study. Free alcohol, intermolecularly and intramolecularly bound alcohol, imine, oxime, ketone, or alkene, stretch phenol, and amine were all identified based on the experimental data. Also, Amin, reach your limits. The peak values for benders, =C-H alkenes, strong C-F alkyl halides, 738.74 strong =C-H alkenes, 813.96 strong =C-H alkenes, 1018.41 strong C-F alkyl halides, and 1415.75 strong C=C aromatic compounds were recorded. The fruit extract (Ethyl acetate, Ethanol, and standards) of Ajwain seeds (Trachyspermum ammi L.) and its antioxidant activities, including peroxynitrite, hydroxyl, and nitric oxide radical scavenging. Crude, ethyl acetate fraction, ethanol fraction, and standard extracts were among the several varieties recorded (725.08±36.07, 617.00±30.41) and Gallic acid (standard) (862.00±38.07) respectively of Peroxynitrite scavenging. While recorded (315.00±28.09, 223.00±21.07), and Mannitol (standard) (618.09±29.14) respectively Hydroxyl radical scavenging potential. At the same time record (48.00±3.04, 21.89±2.05), and Curcumin (standard) (78.00±4.96) respectively Nitric oxide radical scavenging potential. Findings from this study point to Trachyspermum ammi L. as a potential plant source of active chemicals with medicinal use for the treatment of certain fungal diseases.

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References

Kaur C, Kapoor HC. Antioxidant activity and total phenolic content of some Asian vegetables. Int J Food Sci Tech 2002;37:153 62.

Davidson A, Jaine T. The Oxford Companion to Food. USA: Oxford University Press; 2006. 14. Khanuja SP. Formulation comprising thymol useful in the treatment of drug resistance bacterial infection. New Delhi: United State Patent No US 6,824,795 b2, CCIR; 2004.

Javed IM, Akhtar T, Khaliq MZ, Khan G, Muhammad M. Antihyperlipidaemic effect of Trachyspermum ammi (Ajwain) in rabbits. In: Faisalabad: Proc 33rd All Pakistan Science Conference University of Agriculture 2002. p. 80 1.

Gilani AH, Jabeen Q, Ghayur MN, Janbaz KH, Akhtar MS. Studies on the antihypertensive, antispasmodic, bronchodilator and hepatoprotective activities of the Carum copticum seed extract. J Ethnopharmacol 2005;98:127 35.

Ahsan SK, Shah AH, Tanira MO, Ahmad MS, Tariq M, Ageel AM. Studies on some herbal drugs used against kidney stones in Saudi folk medicine. Fitoterapia 1990;61:435 8.

Boskabady MH, Jandaghi P, Kiani S, Hasanzadeh L. Antitussive effect of Carum copticum in guinea pigs. J Ethnopharmacol 2005;97:79 82.

Murthy PS, Borse BB, Khanum H, Srinivas P. Inhibitory effects of Ajwain (Trachyspermum ammi) ethanolic extract on A. orchaceus growth and ochratoxin production. Turk J Biol 2009;33:211 7.

Park IK, Kim J, Lee SG, Shin SC. Nematicidal Activity of Plant Essential Oils and Components From Ajowan (Trachyspermum ammi), Allspice (Pimenta dioica) and Litsea (Litsea cubeba) Essential Oils Against Pine Wood Nematode (Bursaphelenchus Xylophilus). J Nematol 2007;39:275 9.

Alzweiri, M., Al Sarhan, A., Mansi, K., Hudaib, M. & Aburjai, T. Ethnopharmacological survey of medicinal herbs in Jordan, the Northern Badia region. J. Ethno . 137 (1), 27–35 (2011).

Bhardwaj, S. & Gakhar, S. K. Ethnomedicinal plants used by the tribals of Mizoram to cure cuts & wounds. Indian J. Traditional Knowl. 4 (1), 75–80 (2005). 8. Lenski, R. E. Bacterial evolution and the cost of antibiotic resistance. Int. Microbiol. 1 (4), 265–270 (1998).

Raghunath, D. Emerging antibiotic resistance in bacteria with special reference to India. J. Biosci. 33 (4), 593–603 (2008).

Rashi, B. & Sadhna, P. Chemical composition of Indian Ajowan (Carum Coputicum L.) seed oil in Kanpur region of North India. Asian J. Experimental Chem. 5 (1), 31–32 (2010).

Dwivedi, S. N., Mishra, R. P. & Alava, S. Phytochemistry, pharmacological studies and traditional benefits of Trachyspermum ammi (Linn.) Sprague. Int. J. Pharm. life Sci. 3 (5), 1705–1709 (2012).

Rajput, M. A., Khan, R. A., Qazi, N. & Feroz, Z. Effect of methanol extract of ajwain (Trachyspermum ammi L) on blood coagulation in rats. JLUMHS . 11 (02), 105 (2012).

Nickavar, B. & Abolhasani, F. A. Screening of antioxidant properties of seven Umbelliferae fruits from Iran. Pak J. Pharm. Sci. 22 (1), 30–35 (2009).

Hejazian, S. H., Morowatisharifabad, M. & Mahdavi, S. M. Relaxant effect of Carum copticum on intestinal motility in ileum of rat. World J. Zool. 2 (2), 15–18 (2007).

Boskabady, M. & Shaikhi, J. Inhibitory effect of Carum copticum on histamine (H1) receptors of isolated guinea-pig tracheal chains. J. Ethnopharmacol. 69 (3), 217–227 (2000).

Natanzian Ghahfarkhi, M., Sattari, M., Yadegari, M. H., Goudarzi, G. R., & Saharkhiz, M. J. Antifungal activity of essential oil and alcoholic extract of Carum copticum against fluconazole-resistant and susceptible Candida albicans isolated. Pathobiology Res. 11, 0-0 (2008).

Bansod, S. & Rai, M. Antifungal activity of essential oils from Indian medicinal plants against human pathogenic aspergillus fumigatus and A. Niger. World J. Med. Sci. 3 (2), 81–88 (2008).

Rasooli, I. et al. Antimycotoxigenic characteristics of Rosmarinus officinalis and Trachyspermum copticum L. essential oils. Int. J. Food Microbiol. 122 (1), 135–139 (2008).

Mathew N, Misra Bhattacharya S, Perumal V, Muthuswamy K. Antifilarial lead molecules isolated from Trachyspermum ammi. Molecules 2008;13:2156 68.

Aruoma OI, Halliwell B, Hoey BM, Butler J: The antioxidant action of N-acetylcysteine: Its reaction with hydrogen peroxide, hydroxyl radical, superoxide, and hypochlorous acid. Free Rad Biol Med. 1989, 6: 593-597.

Halliwell B, Gutteridge JMC, Aruoma OI: The deoxyribose method: a simple 'test tube' assay for determination of rate constants for reaction of hydroxyl radicals. Anal Biochem. 1987, 165: 215-219.

Miller MJ, Sadowska-Krowicka H, Chotinaruemol S, Kakkis JL, Clark DA: Amelioration of chronic ileitis by nitric oxide synthase inhibition. J Pharmacol Exp Ther. 1993, 264 (1): 11-16.

Balavoine GG, Geletti YV: Peroxynitrite scavenging by different antioxidants. Part 1: convenient study. Nitric oxide. 1999, 3: 40-54.

Beckman JS, Chen H, Ischiropulos H, Crow JP: Oxidative chemistry of peroxynitrite. Methods Enzymol. 1994, 233: 229-240.

Tsimidou M, Boskou D. Antioxidant activity of essential oils from the plants of the Lamiaceae family. Charalambous G, editor. Species, Herbs and Edible Fungi. Amsterdam: Elsevier; 1994. p. 273 8.

Awadhesh K, Mishra RK, Srivastava S, Tiwari AK, Pandey A, ShuklaAC. Role of phylogenetic analysis for anti bacterial activity of essential oil of Trachyspermum ammi L. against water borne pathogens. Adv Environ Res 2011;5:1271 8.

AshnagarA, Naseri G, Ramazani M. Characterization of the major chemical compounds foung in Thymus vulgaris plant grown wildly in Chahar Mahal and Bakhtiari province of Iran. Int J PharmTech Res 2011;3:1 4.

Vilasrao JK, Joshi YM, Sawant HP, Jadhav TA. Free radical scavenging activity of aqueous solution of black salt. Int J Pharm Pharm Sci 2010;2:95 6.

Ruch RJ, Cheng SJ, Klaunig JE. Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis 1989;10:1003 8.

Benzie IF, Strain JJ. Ferric reducing/antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods Enzymol 1999;299:15 27.

CaoG, Alessio HM, CutlerRG. Oxygen radical absorbance capacity assay for antioxidants. Free Radic Biol Med 1993;14:303 11.

Yang J, Guo J, Yuan J. In vitro antioxidant properties of rutin. Lebensm Wiss Technol 2008;41:1060‐6.

Nabavi SM, Ebrahimzadeh MA, Nabavi SF, Jafari M. Free radical scavenging activity and antioxidant capacity of Eryngium caucasicum Trautv and Froripia subpinnata. Pharmacol online 2008;3:19 25.