Detection of Bioactive Natural Products Produced from Bacillus cereus Using Gas Chromatography-Mass Spectroscopy and Evaluation of Its Efficiency of Antibacterial Activity

Abstract

Aims and Objective: This work involved characterizing the bioactive chemical composition of Bacillus cereus and establishing the efficacy of some plant extracts on the bacterium with regard to antibacterial, antifungal, and in-vitro antimicrobial activity.
Method: Bioactives which are chemical compounds that may also be termed as secondary metabolites were analyzed through a (GC-MS) technique. After that, the current investigation evaluated the antibacterial and antibacterial potency of the methanolic extract of Bacillus cereus both in vitro and in vivo.
Results: GC-MS analysis of Bacillus cereus revealed the existence of the: β Carotene , 1-Diisopropylsilyloxy-10-undene , 3-chloro-,(R*,R*)- , 6-Acetyl- β-d-mannose , Thi-eno[2,3-c]-furan-3-carbonitrile, 17-Octadecynoic acid , 3-(3-Carboxy-4-hydrox yphenyl)-D-alanine , N,N'-bis(2- hydroxyl-ethyl)- , Thieno [2,3-c]furan-3- carbnitrile, 2-amino-4, 6-dihydro-4,4,6,6- , 2- (9-octade-cenyl-oxy) ethyl ester , 1,8-Diethyl-3, 6-diazahomoadamantan-9-ol , Lactose , 1-[[3-Methyl-phenyl-]butyryl-4-[5-phenyl-4-oxo-2-oxzolin-2-yl] , 9,10-Seocholesta-5, 7,10(19)-triene3,24, 25-triol,(3β,5Z,7E)-, Ergsta-5, 22-dien-3-ol,ace-tate, (3β,22E)- , Aceta-mide , 1,4- Diacetyl-3-acetoxy-methyl-2, 5-methylene -l-rhamnit-ol , 2-[[2-[[2-[(2-pentyl-cyclo-propyl) methyl , Estra -1,3,5(10)-trien-17β-ol , [1,1'-Bicyclo-propyl]-2-octanoic acid , 5-Hydroxy-methylfufural , β-D-Gluco-pyranoside, methyl , Tetra-acetyld-xylo-nic nitrile , n-Hexa-decanoic acid , 9-Octa-decenoic acid , (2- phenyl-1 ,3-dioxo-lan-4-yl)-methyl ester , Octad-canoic acid , 9,10- Secocho-lesta-5,7,10(19)--triene-3,24,25-triol,(3β,5Z,7E)- and Pyrimidin-2-ol,4-(3,4-dimethoxy-phenyl)-6-phenyl, 2-(3-Hydroxy-propyl)-cyclo-hexane-1 ,3-dione, 1,2- Cyclo-pentanedione,3-methyl, α-D-Gluco-pyrano-side, O-α-D- gluco-pyranosyl-(1.fwdarw.3)-ß-D-fruc, Bicyclo-hep-tane-2- carboxylic acid isobutyl-amide, Cycloun-decanone , 6-Acetyl -ß- d- mannose, 5-Hydroxy-methyl- furfural, D-fructose , Octadecanal ,2 –bromo, L-Ascorbic acid , 6-octad-canoate. Antibacterial activity was assessed Proteus mirabilis was extremely sensitive to the metabolites of Bacillus cereus (7.9±0.04). Evaluation discovered antifungal activity Bacillus cereus metabolites were extremely effective (11.03±0.05) against Cladosporium herbarum. Against Bacillus cereus, Cassia angustifolia (Crude) (12.05 and 11.65 mm) was quite effective.