A Study of Liver Function Tests, Lymphocytes, Neutrophils Count, Phagocytosis Activity and Using the Stain Method for Lymphocyte Transformation Assay in Hepatitis B Viruses Infected Patients

Authors

  • Assist. Prof. Hider M. H. Al-Shirifi Al-Qasim Green University, Faculty of Environmental Sciences, Environmental Health Department, Iraq
  • Assist. Prof. Dr. Hiba Jasim Hamza University of Babylon, College of Nursing, Medical & Basic Sciences Department, Iraq
  • Prof. Dr. Abbas. K. Al-Mansoori AL-Qasim Green University, Faculty of Biotechnology, Department of Applied Biotechnology, Iraq

Keywords:

Hepatitis B Viruses, Lymphocyte Transformation, Phagocytosis Activity, Patients

Abstract

Background: HB is in the list of leading causes of illness and death globally, and in Asia alone, the number of affected people is unnaturally high. Earlier studies have hypothesized that, the virulence factor of HB is modulated by the immune response. The aims of this study were to determine the level of phagocytic cells and the response of T-lymphocyte proliferation in adult patients in Iraq who contracted HBV.
Materials and Methods: These parameters include hepatitis B surface antigen (HBsAg) and other HBV indicators in sixty patients with hepatitis B virus (HBV) serum determined with the use of enzyme-linked immunosorbent assay (EIISA) kits. The basic procedure of obtaining PBMs was by the Ficoll-Hypaque density gradient centrifugation technique. Using flat-bottomed microtiter plates, three sets of cells were cultured: One of them is on a lymphocyte suspension and the other on diluted blood.
Results: The healthy group, than indicated a very significant increase (P<0.05) in the liver function enzymes. Compared with members in the control group, all the patients had a significant decrease (P<0.05) in both the lymphocyte count and the neutrophil count. The present study revealed that the lymphocyte transformation was significantly low in healthy carriers when compared with the controls (P<0.05).

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Published

2024-10-04

How to Cite

Assist. Prof. Hider M. H. Al-Shirifi, Assist. Prof. Dr. Hiba Jasim Hamza, & Prof. Dr. Abbas. K. Al-Mansoori. (2024). A Study of Liver Function Tests, Lymphocytes, Neutrophils Count, Phagocytosis Activity and Using the Stain Method for Lymphocyte Transformation Assay in Hepatitis B Viruses Infected Patients. Current Clinical and Medical Education, 2(10), 44–55. Retrieved from https://www.visionpublisher.info/index.php/ccme/article/view/187

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Vol. 2 No. 10 (2024)

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