Antioxidant (Nitric oxide radical scavenging and Hypochlorous acid scavenging), Antifungal Activity and Investigation of Secondary Metabolites of Alcea Rosea L. Flowers Using GC-MS Technique
Keywords:
Antioxidant, Radical scavenging, Antifungal Activity, Secondary Metabolites, Alcea Rosea LAbstract
Starch, gum, and extraordinarily high content of phenols show that Alcea rosea is an
important medicinal plant. Thirdly, This plant has been used in ancient times to cure all
kinds of ailments and is cultivated for trade. This work aimed at identifying the secondary
metabolites present in Alcea rosea L. flowers using the GC-MS analysis and to assess their
antioxidant activities as nitric oxide radical scavenging, hypochlorous acid scavenging
activities and antifungal activity. At the state-of-the-art Botanical laboratory, the
researchers used dried plantingredients that were purchased from the Babylonian markets.
They were milled using an electrical grinder until reduced to powder form and then put in
nylon bags and left at the laboratory temperature till required. Separation and identification
was done using the GC-MS. GC-MS analysis was performed with fused silica capillary
column, Trace GC Ultra/ISQ Single Quadruple MS Thermo Scientific. The antifungal
activity is assessed using the diameter of the inhibition zone, in millimeters (mm), for each
methanolic extract. Different antioxidant activity of Alcea rosea L. extracted in methanol,
ethanol and standards using nitric oxide radical scavenging the hypochlorous acid
scavenging. Different extract profiles that analysed were Crude, Ethanol fraction and
Standard recorded Nitric oxide radical scavenging 33.45 ± 2.07, 49.00 ± 3.05 and
Curcumin (standard) 87.60 ± 4.13 respectively. For recorded it was 176.00±5.00,
187.33±7.09 and Ascorbic acid (standard) 215.00 ± 8.93 respectively. The scavenging
results demonstrated that crude and other fractions have significantly higher percentage
inhibition against hypochlorous acid activity than the standard Mannitol. These results
confirm other studies for the identification of Alcea rosea as potential plant source for
active compounds for the treatment of some fungal diseases. Antifungal activity of
secondary metabolites of Alcea Rosea L. : Effectiveness of the methanolic crude extract,
ethanol fraction of Alcea rosea L. and standard antibiotics against four fungi and yeast. In
vitro Alcea Rosea L. compounds displayed a high degree of effectiveness against Fusarium
oxyporum ((24.08 ± 0.48 and 15.11 ± 0.49).
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